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Supplementary file1 3D-FISH confocal image captures of each round of hybridization (a, b, c) of a spermatogonia-early preleptotene nucleus. Different combinations of three chromosomes (Chr.) displayed in FITC, Texas Red, or Aqua DEAC are observed in each round. For each hybridization round are shown: first, maximum intensity projections of confocal serial sections in RGB and scale grey; second, slice images segmentation by Fiji; and third, 3D composite reconstructions using Imaris 9.3 software in both RGB merge and split channels.   (MP4 22.6 MB)
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Supplementary file1 3D-FISH confocal image captures of each round of hybridization (a, b, c) of a spermatogonia-early preleptotene nucleus. Different combinations of three chromosomes (Chr.) displayed in FITC, Texas Red, or Aqua DEAC are observed in each round. For each hybridization round are shown: first, maximum intensity projections of confocal serial sections in RGB and scale grey; second, slice images segmentation by Fiji; and third, 3D composite reconstructions using Imaris 9.3 software in both RGB merge and split channels.   (MP4 22.6 MB)
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Supplementary file1 3D-FISH confocal image captures of each round of hybridization (a, b, c) of a spermatogonia-early preleptotene nucleus. Different combinations of three chromosomes (Chr.) displayed in FITC, Texas Red, or Aqua DEAC are observed in each round. For each hybridization round are shown: first, maximum intensity projections of confocal serial sections in RGB and scale grey; second, slice images segmentation by Fiji; and third, 3D composite reconstructions using Imaris 9.3 software in both RGB merge and split channels.   (MP4 22.6 MB)
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Supplementary file1 3D-FISH confocal image captures of each round of hybridization (a, b, c) of a spermatogonia-early preleptotene nucleus. Different combinations of three chromosomes (Chr.) displayed in FITC, Texas Red, or Aqua DEAC are observed in each round. For each hybridization round are shown: first, maximum intensity projections of confocal serial sections in RGB and scale grey; second, slice images segmentation by Fiji; and third, 3D composite reconstructions using Imaris 9.3 software in both RGB merge and split channels.   (MP4 22.6 MB)
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Image Search Results


Supplementary file1 3D-FISH confocal image captures of each round of hybridization (a, b, c) of a spermatogonia-early preleptotene nucleus. Different combinations of three chromosomes (Chr.) displayed in FITC, Texas Red, or Aqua DEAC are observed in each round. For each hybridization round are shown: first, maximum intensity projections of confocal serial sections in RGB and scale grey; second, slice images segmentation by Fiji; and third, 3D composite reconstructions using Imaris 9.3 software in both RGB merge and split channels.   (MP4 22.6 MB)

Journal: Chromosoma

Article Title: Time to match; when do homologous chromosomes become closer?

doi: 10.1007/s00412-022-00777-0

Figure Lengend Snippet: Supplementary file1 3D-FISH confocal image captures of each round of hybridization (a, b, c) of a spermatogonia-early preleptotene nucleus. Different combinations of three chromosomes (Chr.) displayed in FITC, Texas Red, or Aqua DEAC are observed in each round. For each hybridization round are shown: first, maximum intensity projections of confocal serial sections in RGB and scale grey; second, slice images segmentation by Fiji; and third, 3D composite reconstructions using Imaris 9.3 software in both RGB merge and split channels.   (MP4 22.6 MB)

Article Snippet: For each hybridization round are shown maximum intensity projections of confocal serial sections and 3D composite reconstructions using Imaris 9.3 software in both RGB merge and split channels (to view the planes’ sequence of the maximum intensity confocal images and the 3D composite reconstruction of premeiotic cells, see Online resource ) Image analysis was performed using customized developed scripts designed within the Fiji software environment (Schindelin et al. ) and Matlab R2013b.

Techniques: